WEE1 Antikörper (WEE1 Homolog (S. Pombe)) (pSer139)

Details for Product anti-WEE1 Antibody No. ABIN6269995
Target
Bindungsspezifität
pSer139
25
16
16
15
15
15
14
12
10
10
10
7
4
4
3
2
2
2
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
157
83
71
2
1
Wirt
Kaninchen
151
7
2
Klonalität
Polyklonal
Konjugat
Dieser WEE1 Antikörper ist unkonjugiert
14
12
11
8
8
8
5
5
5
5
5
5
2
2
2
2
2
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
103
86
56
28
15
15
4
4
3
3
1
1
Optionen
Immunogen A synthesized peptide derived from human WEE1 around the phosphorylation site of Ser139
Isotyp IgG
Spezifität Phospho-WEE1 (Ser139) Antibody detects endogenous levels of WEE1 only when phosphorylated at Ser139
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung WEE1 (WEE1 Antibody Abstract)
Hintergrund

Description: Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

Gene: WEE1

Molekulargewicht 71kDa
Gen-ID 7465
UniProt P30291
Pathways Zellzyklus, Mitotic G1-G1/S Phases, M Phase
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-WEE1 Homolog (S. Pombe) (WEE1) (pSer139) antibody (ABIN6269995) ABIN6269996 at 1/100 staining human gastric cancer tissue sections by IHC-P. The tiss...
Image no. 2 for anti-WEE1 Homolog (S. Pombe) (WEE1) (pSer139) antibody (ABIN6269995) ABIN6269996 at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was for...
Image no. 3 for anti-WEE1 Homolog (S. Pombe) (WEE1) (pSer139) antibody (ABIN6269995) Western blot analysis of Phospho-WEE1 (Ser139) in lysates of 293 etoposide 25uM 60', ...