DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (pSer84) Antikörper

Details zu Produkt Nr. ABIN6269967
Target
Bindungsspezifität
pSer84
54
27
25
20
16
16
15
15
15
13
9
8
7
7
7
4
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
309
144
59
53
49
3
2
2
2
1
1
1
1
1
1
Wirt
Kaninchen
208
91
7
5
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
22
17
16
11
11
11
10
9
9
9
9
9
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
187
90
79
66
57
53
38
35
30
26
25
5
4
3
3
2
2
1
1
1
1
Optionen
Immunogen A synthesized peptide derived from human DNMT1 around the phosphorylation site of Ser84
Isotyp IgG
Spezifität Phospho-DNMT1 (Ser84) Antibody detects endogenous levels of DNMT1 only when phosphorylated at Ser84
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung DNMT1 (DNMT1 Antibody Abstract)
Hintergrund

Description: Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). Promotes tumor growth (PubMed:24623306).

Gene: DNMT1

Molekulargewicht 183kDa
Gen-ID 1786
UniProt P26358
Pathways SARS-CoV-2 Protein Interaktom, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (pSer84) antibody (ABIN6269967) Western blot analysis of Phospho-DNMT1 (Ser84) in lysates of HCT116, using Phospho-DN...
Image no. 2 for anti-DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) (pSer84) antibody (ABIN6269967) ABIN6269968 at 1/100 staining rat brain tissue sections by IHC-P. The tissue was form...