RNA Polymerase II CTD (pTyr145) Antikörper

Details zu Produkt Nr. ABIN6269959
Target
Bindungsspezifität
pTyr145
16
11
7
5
4
3
2
2
1
1
1
1
Reaktivität
Human, Maus, Ratte
45
27
20
4
4
2
2
1
1
1
1
1
Wirt
Kaninchen
27
13
5
Klonalität
Polyklonal
Konjugat
Unkonjugiert
2
2
2
2
2
2
2
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
34
21
18
16
9
9
8
7
4
2
1
Optionen
Immunogen A synthesized peptide derived from human Rpb1 CTD around the phosphorylation site of Tyr145
Isotyp IgG
Spezifität Phospho-Rpb1 CTD (Tyr145) Antibody detects endogenous levels of Rpb1 CTD only when phosphorylated at Tyr145
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung Rpb1 CTD
Hintergrund

Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Regulation of gene expression levels depends on the balance between methylation and acetylation levels of tha CTD-lysines (By similarity). Initiation or early elongation steps of transcription of growth-factors-induced immediate early genes are regulated by the acetylation status of the CTD (PubMed:24207025). Methylation and dimethylation have a repressive effect on target genes expression (By similarity).

Gene: POLR2A

Molekulargewicht 270kDa
Gen-ID 5430
UniProt P24928
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-RNA Polymerase II CTD (pTyr145) antibody (ABIN6269959) ABIN6269960 at 1/100 staining rat heart tissue sections by IHC-P. The tissue was form...
Image no. 2 for anti-RNA Polymerase II CTD (pTyr145) antibody (ABIN6269959) Western blot analysis of Phospho-Rpb1 CTD (Tyr145) in lysates of COS7 EGF 200ng/ml 30...
Image no. 3 for anti-RNA Polymerase II CTD (pTyr145) antibody (ABIN6269959) ABIN6269960 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was f...
Image no. 4 for anti-RNA Polymerase II CTD (pTyr145) antibody (ABIN6269959) ABIN6269960 at 1/100 staining mouse liver tissue sections by IHC-P. The tissue was fo...