PKA CAT (pThr197) Antikörper

Details zu Produkt Nr. ABIN6269905
Target
Bindungsspezifität
pThr197
7
1
1
Reaktivität
Human, Maus, Ratte
11
11
11
Wirt
Kaninchen
11
Klonalität
Polyklonal
Konjugat
Unkonjugiert
1
1
1
1
1
1
1
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
6
5
3
3
1
1
1
Optionen
Immunogen A synthesized peptide derived from human PKA CAT around the phosphorylation site of Thr197
Isotyp IgG
Spezifität Phospho-PKA CAT (Thr197) Antibody detects endogenous levels of PKA CAT only when phosphorylated at Thr197
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Hintergrund

Description: Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen, maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca2+, leading to reduced amplitude and increased frequency of store overload-induced Ca2+ release (SOICR) characterized by an increased rate of Ca2+ release and propagation velocity of spontaneous Ca2+ waves, despite reduced wave amplitude and resting cytosolic Ca2+. PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Isoform 2 phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation. Phosphorylates HSF1, this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock (PubMed:21085490).

Gene: PRKACA

Molekulargewicht 40kDa
Gen-ID 5566
UniProt P17612, P22694, P22612
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-PKA CAT (pThr197) antibody (ABIN6269905) ABIN6269906 at 1/100 staining mouse heart tissue sections by IHC-P. The tissue was fo...
Image no. 2 for anti-PKA CAT (pThr197) antibody (ABIN6269905) Western blot analysis of extracts of mouse brain tissue sample,using Phospho-PKA CAT ...
Image no. 3 for anti-PKA CAT (pThr197) antibody (ABIN6269905) ABIN6269906 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was f...
Image no. 4 for anti-PKA CAT (pThr197) antibody (ABIN6269905) ABIN6269906 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was for...