HCK Antikörper (Hemopoietic Cell Kinase) (pTyr522)

Details for Product anti-HCK Antibody No. ABIN6269835
Target
Bindungsspezifität
pTyr522
19
10
9
8
7
7
7
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
93
42
29
1
1
1
1
1
Wirt
Kaninchen
82
11
1
Klonalität
Polyklonal
Konjugat
Dieser HCK Antikörper ist unkonjugiert
7
6
6
5
5
5
2
1
1
1
1
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
75
54
29
17
13
12
7
3
2
2
1
1
1
Optionen
Immunogen A synthesized peptide derived from human HCK around the phosphorylation site of Tyr522
Isotyp IgG
Spezifität Phospho-HCK (Tyr522) Antibody detects endogenous levels of HCK only when phosphorylated at Tyr522
Kreuzreaktivität Human
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung HCK (HCK Antibody Abstract)
Hintergrund

Description: Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.

Gene: HCK

Molekulargewicht 59kDa
Gen-ID 3055
UniProt P08631
Pathways Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Hemopoietic Cell Kinase (HCK) (pTyr522) antibody (ABIN6269835) Western blot analysis of Phospho-HCK (Tyr522) in lysates of Jurkat, using Phospho-HCK...