IGF1R Antikörper (Insulin-Like Growth Factor 1 Receptor) (pThr1163)

Details for Product anti-IGF1R Antibody No. ABIN6269831
Target
Bindungsspezifität
pThr1163
28
22
22
21
20
19
16
15
15
15
15
13
12
11
8
7
7
7
7
7
5
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
332
202
200
15
13
12
11
9
5
4
4
3
2
2
1
Wirt
Kaninchen
277
53
2
2
1
Klonalität
Polyklonal
Konjugat
Dieser IGF1R Antikörper ist unkonjugiert
19
16
13
13
11
11
10
10
10
10
8
8
8
8
8
5
4
1
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
191
104
97
84
60
60
33
31
18
6
6
6
5
3
2
1
1
Optionen
Immunogen A synthesized peptide derived from human IGF-I Receptor around the phosphorylation site of Thr1163
Isotyp IgG
Spezifität Phospho-IGF-I Receptor (Thr1163) Antibody detects endogenous levels of IGF-I Receptor only when phosphorylated at Thr1163
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung IGF-I Receptor (IGF1R Antibody Abstract)
Hintergrund

Description: Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R.

Gene: IGF1R

Molekulargewicht 96kDa
Gen-ID 3480
UniProt P08069
Pathways RTK Signalweg, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagie
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (pThr1163) antibody (ABIN6269831) ABIN6269832 at 1/100 staining rat lung tissue sections by IHC-P. The tissue was forma...
Image no. 2 for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (pThr1163) antibody (ABIN6269831) Western blot analysis of Phospho-IGF-I Receptor (Thr1163) in lysates of 293 Insulin,...
Image no. 3 for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (pThr1163) antibody (ABIN6269831) ABIN6269832 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was f...
Image no. 4 for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (pThr1163) antibody (ABIN6269831) ABIN6269832 at 1/100 staining mouse heart tissue sections by IHC-P. The tissue was fo...