HSP90AA1 Antikörper (Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1) (pTyr197)

Details for Product anti-HSP90AA1 Antibody No. ABIN6269827
Target
Bindungsspezifität
pTyr197
8
7
5
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
96
74
62
17
16
13
13
11
10
9
7
7
5
5
5
2
1
1
1
1
1
Wirt
Kaninchen
67
30
4
1
Klonalität
Polyklonal
Konjugat
Dieser HSP90AA1 Antikörper ist unkonjugiert
9
3
2
2
2
2
2
2
2
1
1
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
89
33
29
26
23
20
17
6
4
3
2
1
1
Optionen
Immunogen A synthesized peptide derived from human HSP90A around the phosphorylation site of Tyr197
Isotyp IgG
Spezifität Phospho-HSP90A (Tyr197) Antibody detects endogenous levels of HSP90A only when phosphorylated at Tyr197
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung HSP90A (HSP90AA1 Antibody Abstract)
Hintergrund

Description: Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (PubMed:11274138, PubMed:15577939, PubMed:15937123, PubMed:27353360, PubMed:29127155). Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself (PubMed:29127155). Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle (PubMed:27295069, PubMed:26991466). Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels (PubMed:25973397). In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues(PubMed:25973397). Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment (PubMed:25973397). Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression (PubMed:25973397). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11276205). Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation (PubMed:24613385).

Gene: HSP90AA1

Molekulargewicht 90kDa
Gen-ID 3320
UniProt P07900
Pathways M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) (pTyr197) antibody (ABIN6269827) Western blot analysis of Phospho-HSP90A (Tyr197) in lysates of NIH-3T3, using Phospho...
Image no. 2 for anti-Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) (pTyr197) antibody (ABIN6269827) ABIN6269828 at 1/100 staining rat lung tissue sections by IHC-P. The tissue was forma...
Image no. 3 for anti-Heat Shock Protein 90kDa alpha (Cytosolic), Class A Member 1 (HSP90AA1) (pTyr197) antibody (ABIN6269827) ABIN6269828 at 1/100 staining mouse brain tissue sections by IHC-P. The tissue was fo...