EPRS Antikörper (Glutamyl-Prolyl-tRNA Synthetase) (pTyr377)

Details for Product anti-EPRS Antibody No. ABIN6269825
Target
Bindungsspezifität
pTyr377
7
7
4
3
3
2
2
2
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
54
40
25
4
4
3
2
2
2
2
1
1
1
Wirt
Kaninchen
56
Klonalität
Polyklonal
Konjugat
Dieser EPRS Antikörper ist unkonjugiert
5
4
4
3
3
3
3
2
2
1
1
1
1
1
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
29
13
12
10
5
5
3
3
2
2
1
Optionen
Immunogen A synthesized peptide derived from human EPRS around the phosphorylation site of Tyr377
Isotyp IgG
Spezifität Phospho-EPRS (Tyr377) Antibody detects endogenous levels of EPRS only when phosphorylated at Tyr377
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung EPRS (EPRS Antibody Abstract)
Hintergrund

Description: Multifunctional protein which is primarily part of the aminoacyl-tRNA synthetase multienzyme complex, also know as multisynthetase complex, that catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA (PubMed:1756734, PubMed:24100331, PubMed:23263184). The phosphorylation of EPRS, induced by interferon-gamma, dissociates the protein from the aminoacyl-tRNA synthetase multienzyme complex and recruits it to the GAIT complex that binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin), suppressing their translation. Interferon-gamma can therefore redirect, in specific cells, the EPRS function from protein synthesis to translation inhibition (PubMed:15479637, PubMed:23071094). Also functions as an effector of the mTORC1 signaling pathway by promoting, through SLC27A1, the uptake of long-chain fatty acid by adipocytes. Thereby, it also plays a role in fat metabolism and more indirectly influences lifespan (PubMed:28178239).

Gene: EPRS

Molekulargewicht 171kDa
Gen-ID 2058
UniProt P07814
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Glutamyl-Prolyl-tRNA Synthetase (EPRS) (pTyr377) antibody (ABIN6269825) ABIN6269826 at 1/100 staining human brain tissue sections by IHC-P. The tissue was fo...
Image no. 2 for anti-Glutamyl-Prolyl-tRNA Synthetase (EPRS) (pTyr377) antibody (ABIN6269825) ABIN6269826 at 1/100 staining rat lung tissue sections by IHC-P. The tissue was forma...
Image no. 3 for anti-Glutamyl-Prolyl-tRNA Synthetase (EPRS) (pTyr377) antibody (ABIN6269825) ABIN6269826 at 1/100 staining mouse heart tissue sections by IHC-P. The tissue was fo...
Image no. 4 for anti-Glutamyl-Prolyl-tRNA Synthetase (EPRS) (pTyr377) antibody (ABIN6269825) Western blot analysis of Phospho-EPRS (Tyr377) in lysates of Jurkat?, using Phospho-E...