Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (pSer10) Antikörper

Details zu Produkt Nr. ABIN6269819
Target
Bindungsspezifität
pSer10
24
18
16
16
16
12
9
8
7
7
6
5
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
209
128
122
17
13
12
10
9
9
8
5
3
2
2
1
1
1
1
1
Wirt
Kaninchen
169
57
8
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
17
7
7
6
6
6
6
6
5
3
3
3
3
3
2
2
2
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
171
92
85
53
50
40
23
21
14
12
12
8
5
5
3
3
Optionen
Immunogen A synthesized peptide derived from human NPM around the phosphorylation site of Ser10
Isotyp IgG
Spezifität Phospho-NPM (Ser10) Antibody detects endogenous levels of NPM only when phosphorylated at Ser10
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung NPM (NPM1 Antibody Abstract)
Hintergrund

Description: Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).

Gene: NPM1

Molekulargewicht 37kDa
Gen-ID 4869
UniProt P06748
Pathways Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (pSer10) antibody (ABIN6269819) ABIN6269820 at 1/100 staining rat lung tissue sections by IHC-P. The tissue was forma...
Image no. 2 for anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (pSer10) antibody (ABIN6269819) ABIN6269820 at 1/100 staining mouse heart tissue sections by IHC-P. The tissue was fo...
Image no. 3 for anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (pSer10) antibody (ABIN6269819) ABIN6269820 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was f...
Image no. 4 for anti-Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1) (pSer10) antibody (ABIN6269819) Western blot analysis of Phospho-NPM (Ser10) in lysates of HeLa?, using Phospho-NPM (...