EIF4E Antikörper (Eukaryotic Translation Initiation Factor 4E) (pThr210)

Produktnummer ABIN6269817

Produktdetails anti-EIF4E Antikörper

Target:

EIF4E

Bindungsspezifität: pThr210

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF4E Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Spezifität: Phospho-eIF4E (Thr210) Antibody detects endogenous levels of eIF4E only when phosphorylated at Thr210

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Aufreinigung: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human eIF4E around the phosphorylation site of Thr210

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:2000, IHC 1:50-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C.Stable for 12 months from date of receipt

Haltbarkeit: 12 months

Details zu EIF4E

Target:

EIF4E

Andere Bezeichnung: eIF4E (EIF4E Antibody Abstract)

Hintergrund:

Description: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.

Gene: EIF4E

Molekulargewicht: 25kDa

Gen-ID: 1977

UniProt: P06730