FYN Antikörper (FYN Oncogene Related To SRC, FGR, YES) (pTyr213)

Details for Product anti-FYN Antibody No. ABIN6269809
Target
Bindungsspezifität
pTyr213, pTyr214
23
11
11
9
9
9
7
7
7
6
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
138
93
53
6
4
4
4
4
4
4
3
2
2
1
Wirt
Kaninchen
115
34
8
Klonalität
Polyklonal
Konjugat
Dieser FYN Antikörper ist unkonjugiert
11
8
7
7
6
6
3
3
3
3
3
3
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
134
87
40
18
14
13
13
7
3
3
2
2
1
Optionen
Immunogen A synthesized peptide derived from human Fyn around the phosphorylation site of Tyr213+214
Isotyp IgG
Spezifität Phospho-Fyn (Tyr213+214) Antibody detects endogenous levels of Fyn only when phosphorylated at Tyr213+214
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung Fyn (FYN Antibody Abstract)
Hintergrund

Description: Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1.

Gene: FYN

Molekulargewicht 61kDa
Gen-ID 2534
UniProt P06241
Pathways JAK-STAT Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (pTyr213), (pTyr214) antibody (ABIN6269809) Western blot analysis of Phospho-Fyn (Tyr213+214) in lysates of 293 H2O2 100uM 15', u...
Image no. 2 for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (pTyr213), (pTyr214) antibody (ABIN6269809) ABIN6269810 at 1/100 staining rat lung tissue sections by IHC-P. The tissue was forma...
Image no. 3 for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (pTyr213), (pTyr214) antibody (ABIN6269809) ABIN6269810 at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was for...
Image no. 4 for anti-FYN Oncogene Related To SRC, FGR, YES (FYN) (pTyr213), (pTyr214) antibody (ABIN6269809) ABIN6269810 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was f...