LCK Antikörper (Lymphocyte-Specific Protein tyrosine Kinase) (pTyr192)

Details for Product anti-LCK Antibody No. ABIN6269805
Target
Bindungsspezifität
pTyr192
31
22
13
11
10
10
9
9
7
7
7
6
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
221
139
112
11
10
8
7
4
4
4
3
2
1
Wirt
Kaninchen
188
26
8
Klonalität
Polyklonal
Konjugat
Dieser LCK Antikörper ist unkonjugiert
13
9
8
6
6
6
5
4
4
4
4
4
2
2
2
2
2
1
1
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
175
102
53
32
29
26
25
21
14
11
4
3
2
2
1
1
Optionen
Immunogen A synthesized peptide derived from human Lck around the phosphorylation site of Tyr192
Isotyp IgG
Spezifität Phospho-Lck (Tyr192) Antibody detects endogenous levels of Lck only when phosphorylated at Tyr192
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung Lck (LCK Antibody Abstract)
Hintergrund

Description: Non-receptor tyrosine-protein kinase that plays an essential role in the selection and maturation of developing T-cells in the thymus and in the function of mature T-cells. Plays a key role in T-cell antigen receptor (TCR)-linked signal transduction pathways. Constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, thereby recruiting the associated LCK protein to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR-gamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. Once stimulated, the TCR recruits the tyrosine kinase ZAP70, that becomes phosphorylated and activated by LCK. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. LCK also contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, which leads to hyperphosphorylation and activation of LCK. Also plays a role in the IL2 receptor-linked signaling pathway that controls the T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR. Phosphorylates other substrates including RUNX3, PTK2B/PYK2, the microtubule-associated protein MAPT, RHOH or TYROBP. Interacts with FYB2 (PubMed:27335501).

Gene: LCK

Molekulargewicht 56kDa
Gen-ID 3932
UniProt P06239
Pathways T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (pTyr192) antibody (ABIN6269805) ABIN6269806 at 1/100 staining rat heart tissue sections by IHC-P. The tissue was form...
Image no. 2 for anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (pTyr192) antibody (ABIN6269805) ABIN6269806 at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was for...
Image no. 3 for anti-Lymphocyte-Specific Protein tyrosine Kinase (LCK) (pTyr192) antibody (ABIN6269805) Western blot analysis of Phospho-Lck (Tyr192) in lysates of Jurkat, using Phospho-Lck...