Kinase C-Binding Protein 1 (PKCB1) (pThr642), (pThr644) Antikörper

Details zu Produkt Nr. ABIN6269797
Target
Bindungsspezifität
pThr642, pThr644
7
7
1
1
Reaktivität
Human, Maus, Ratte
19
9
8
Wirt
Kaninchen
19
Klonalität
Polyklonal
Konjugat
Unkonjugiert
2
2
2
2
2
2
2
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
9
7
2
2
1
1
Optionen
Immunogen A synthesized peptide derived from human PKCB1 around the phosphorylation site of Thr642+644
Isotyp IgG
Spezifität Phospho-PKCB1(Thr642+644) Antibody detects endogenous levels of PKCB1 only when phosphorylated at Thr642+644
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung PKCB1
Hintergrund

Description: Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription.

Gene: PRKCB

Molekulargewicht 77kDa
Gen-ID 5579
UniProt P05771
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Kinase C-Binding Protein 1 (PKCB1) (pThr642), (pThr644) antibody (ABIN6269797) Western blot analysis of Phospho-PKCB1(Thr642+644) in lysates of HeLa heat shock, us...
Image no. 2 for anti-Kinase C-Binding Protein 1 (PKCB1) (pThr642), (pThr644) antibody (ABIN6269797) ABIN6269798 at 1/100 staining mouse spleen tissue sections by IHC-P. The tissue was f...
Image no. 3 for anti-Kinase C-Binding Protein 1 (PKCB1) (pThr642), (pThr644) antibody (ABIN6269797) ABIN6269798 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was f...
Image no. 4 for anti-Kinase C-Binding Protein 1 (PKCB1) (pThr642), (pThr644) antibody (ABIN6269797) ABIN6269798 at 1/100 staining rat lung tissue sections by IHC-P. The tissue was forma...