p53 Antikörper (Tumor Protein P53) (pTyr327) Primary Antibody
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- Target
- Bindungsspezifität
- pTyr327
- Reaktivität
- Human, Maus, Ratte
- Wirt
- Kaninchen
- Klonalität
- Polyklonal
- Konjugat
- Dieser p53 Antikörper ist unkonjugiert
- Applikation
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Spezifität
- Phospho-p53 (Tyr327) Antibody detects endogenous levels of p53 only when phosphorylated at Tyr327
- Kreuzreaktivität
- Human, Maus, Ratte (Rattus)
- Aufreinigung
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
- Immunogen
- A synthesized peptide derived from human p53 around the phosphorylation site of Tyr327
- Isotyp
- IgG
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-
- Applikationshinweise
- WB 1:500-1:2000, IHC 1:50-1:200
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store at -20 °C.Stable for 12 months from date of receipt
- Haltbarkeit
- 12 months
-
- Target
- Andere Bezeichnung
- p53 (TP53 Antibody Abstract)
- Synonyme
- BCC7, LFS1, P53, TRP53, p53, Trp53, brp53, drp53, etID22686.5, fb40d06, wu:fb40d06, zgc:111919, Tp53, bbl, bfy, bhy, p44, tp53, Xp53, TP53, tumor protein p53, cellular tumor antigen p53, transformation related protein 53, tumor protein p53 L homeolog, TP53, CpipJ_CPIJ002758, Tp53, tp53, Trp53, tp53.L
- Hintergrund
Description: Acts as a tumor suppressor in many tumor types, induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis, the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).
Gene: TP53
- Molekulargewicht
- 43.7kDa
- Gen-ID
- 7157
- UniProt
- P04637
- Pathways
- p53 Signalweg, MAPK Signalweg, PI3K-Akt Signalweg, Apoptose, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagie