RPS6KA5 Antikörper (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5) (pSer211)

Details for Product anti-RPS6KA5 Antibody No. ABIN6269727
Target
Bindungsspezifität
pSer211
32
28
25
22
15
7
5
5
5
4
4
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
196
142
59
8
5
5
5
5
5
1
Wirt
Kaninchen
188
7
2
1
Klonalität
Polyklonal
Konjugat
Dieser RPS6KA5 Antikörper ist unkonjugiert
10
8
7
7
7
7
7
7
6
4
4
4
4
4
2
1
1
1
Applikation
ELISA, Western Blotting (WB)
112
71
53
52
41
33
9
7
7
3
3
3
2
1
Optionen
Immunogen A synthesized peptide derived from human MSK1 around the phosphorylation site of Ser211
Isotyp IgG
Spezifität Phospho-MSK1 (Ser211) Antibody detects endogenous levels of MSK1 only when phosphorylated at Ser211
Kreuzreaktivität Human, Maus
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung MSK1 (RPS6KA5 Antibody Abstract)
Hintergrund

Description: Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes (PubMed:11909979, PubMed:12569367, PubMed:12763138, PubMed:9687510, PubMed:18511904, PubMed:9873047). Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin (PubMed:11909979, PubMed:9873047). Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression (PubMed:12628924, PubMed:18511904). In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress (PubMed:12628924). In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential (PubMed:12763138). Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation (PubMed:12569367). Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A (PubMed:15010469). Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN (PubMed:12773393). May also phosphorylate 'Ser-28' of histone H3 (PubMed:12773393). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14) (PubMed:12773393). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines (By similarity). Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors (By similarity). Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury (By similarity). Phosphorylates TRIM7 at 'Ser-107' in response to growth factor signaling via the MEK/ERK pathway, thereby stimulating its ubiquitin ligase activity (PubMed:25851810).

Gene: RPS6KA5

Molekulargewicht 90kDa
Gen-ID 9252
UniProt O75582
Pathways MAPK Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 5 (RPS6KA5) (pSer211) antibody (ABIN6269727) Western blot analysis of Phospho-MSK1 (Ser211) in lysates of 3T3 EGF 200ng/ml 5 mins,...