ERN1 Antikörper (Endoplasmic Reticulum To Nucleus Signaling 1) (pSer724)

Details for Product anti-ERN1 Antibody No. ABIN6269717
Target
Bindungsspezifität
pSer724
23
15
15
15
8
8
7
7
7
7
7
7
6
4
4
4
4
3
3
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
132
68
62
1
Wirt
Kaninchen
123
13
7
3
Klonalität
Polyklonal
Konjugat
Dieser ERN1 Antikörper ist unkonjugiert
13
10
7
7
7
7
7
7
6
5
5
5
2
2
2
2
2
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
91
69
26
17
16
8
8
7
6
5
3
2
1
Optionen
Immunogen A synthesized peptide derived from human IRE1 around the phosphorylation site of phospho S724
Isotyp IgG
Spezifität Phospho-IRE1 (phospho S724) Antibody detects endogenous levels of IRE1 only when phosphorylated at phospho S724
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung IRE1 (ERN1 Antibody Abstract)
Hintergrund

Description: Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11779464, PubMed:11175748, PubMed:12637535, PubMed:9637683, PubMed:21317875). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed:21317875). Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11779464, PubMed:24508390, PubMed:21317875). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11779464, PubMed:24508390, PubMed:21317875).

Gene: ERN1

Molekulargewicht 110kDa
Gen-ID 2081
UniProt O75460, Q76MJ5
Pathways ER-Nucleus Signaling, Unfolded protein response
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (pSer724) antibody (ABIN6269717) ABIN6269718 at 1/100 staining mouse brain tissue sections by IHC-P. The tissue was fo...
Image no. 2 for anti-Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (pSer724) antibody (ABIN6269717) Western blot analysis of Phospho-IRE1 (phospho S724) in lysates of K562?, using Phosp...
Image no. 3 for anti-Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (pSer724) antibody (ABIN6269717) ABIN6269718 at 1/100 staining human lung tissue sections by IHC-P. The tissue was for...
Image no. 4 for anti-Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (pSer724) antibody (ABIN6269717) ABIN6269718 at 1/100 staining rat spleen tissue sections by IHC-P. The tissue was for...