MITF Antikörper (Microphthalmia-Associated Transcription Factor) (pSer516)

Details for Product anti-MITF Antibody No. ABIN6269699
Target
Bindungsspezifität
pSer516
25
14
10
9
8
8
7
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
181
82
47
30
7
6
5
5
5
5
5
3
3
3
3
2
2
2
Wirt
Kaninchen
110
70
1
1
Klonalität
Polyklonal
Konjugat
Dieser MITF Antikörper ist unkonjugiert
10
6
6
5
5
5
5
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
90
76
71
70
45
29
16
13
7
7
6
6
4
3
3
3
2
1
Optionen
Immunogen A synthesized peptide derived from human MITF around the phosphorylation site of Ser516
Isotyp IgG
Spezifität Phospho-MITF (Ser516) Antibody detects endogenous levels of MITF only when phosphorylated at Ser516
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung MITF (MITF Antibody Abstract)
Hintergrund

Description: Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

Gene: MITF

Molekulargewicht 52kDa
Gen-ID 4286
UniProt O75030
Pathways Chromatin Binding
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Microphthalmia-Associated Transcription Factor (MITF) (pSer516) antibody (ABIN6269699) ABIN6269700 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was f...
Image no. 2 for anti-Microphthalmia-Associated Transcription Factor (MITF) (pSer516) antibody (ABIN6269699) ABIN6269700 at 1/100 staining rat lung tissue sections by IHC-P. The tissue was forma...
Image no. 3 for anti-Microphthalmia-Associated Transcription Factor (MITF) (pSer516) antibody (ABIN6269699) Western blot analysis of Phospho-MITF (Ser516) in lysates of COS7, using Phospho-MITF...