Nibrin Antikörper (NBN) (pSer432)

Details for Product anti-NBN Antibody No. ABIN6269695
Target
Bindungsspezifität
pSer432
33
15
14
9
7
6
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
158
82
60
2
1
1
1
1
Wirt
Kaninchen
144
17
Klonalität
Polyklonal
Konjugat
Dieser Nibrin Antikörper ist unkonjugiert
10
7
6
6
6
6
6
5
3
2
2
2
2
2
2
2
2
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
113
54
39
33
26
24
23
13
11
5
4
2
2
2
1
1
Optionen
Immunogen A synthesized peptide derived from human p95/NBS1 around the phosphorylation site of Ser432
Isotyp IgG
Spezifität Phospho-p95/NBS1 (Ser432) Antibody detects endogenous levels of p95/NBS1 only when phosphorylated at Ser432
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung p95/NBS1 (NBN Antibody Abstract)
Hintergrund

Description: Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.

Gene: NBN

Molekulargewicht 85kDa
Gen-ID 4683
UniProt O60934
Pathways DNA Reparatur, Production of Molecular Mediator of Immune Response
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Nibrin (NBN) (pSer432) antibody (ABIN6269695) ABIN6269696 at 1/100 staining mouse brain tissue sections by IHC-P. The tissue was fo...
Image no. 2 for anti-Nibrin (NBN) (pSer432) antibody (ABIN6269695) ABIN6269696 at 1/100 staining rat lung tissue sections by IHC-P. The tissue was forma...
Image no. 3 for anti-Nibrin (NBN) (pSer432) antibody (ABIN6269695) ABIN6269696 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was f...
Image no. 4 for anti-Nibrin (NBN) (pSer432) antibody (ABIN6269695) Western blot analysis of Phospho-p95/NBS1 (Ser432) in lysates of HuvEc Forskolin 40nM...