RIP2 Antikörper (ROP Interactive Partner 2) (pTyr176)

Details for Product anti-RIP2 Antibody No. ABIN6269655
Target
Bindungsspezifität
pTyr176
17
7
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
34
31
19
Wirt
Kaninchen
32
2
Klonalität
Polyklonal
Konjugat
Dieser RIP2 Antikörper ist unkonjugiert
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
17
14
8
7
3
2
1
1
1
1
Optionen
Immunogen A synthesized peptide derived from human RIP2 around the phosphorylation site of Tyr176
Isotyp IgG
Spezifität Phospho-RIP2 (Tyr176) Antibody detects endogenous levels of RIP2 only when phosphorylated at Tyr176
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung RIP2
Hintergrund

Description: Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation.

Gene: RIPK2

Molekulargewicht 70kDa
Gen-ID 8767
UniProt O43353
Pathways T-Zell Rezeptor Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-ROP Interactive Partner 2 (RIP2) (pTyr176) antibody (ABIN6269655) Western blot analysis of Phospho-RIP2 (Tyr176) in lysates of 293, using Phospho-RIP2 ...
Image no. 2 for anti-ROP Interactive Partner 2 (RIP2) (pTyr176) antibody (ABIN6269655) ABIN6269656 at 1/100 staining human brain tissue sections by IHC-P. The tissue was fo...
Image no. 3 for anti-ROP Interactive Partner 2 (RIP2) (pTyr176) antibody (ABIN6269655) ABIN6269656 at 1/100 staining rat heart tissue sections by IHC-P. The tissue was form...
Image no. 4 for anti-ROP Interactive Partner 2 (RIP2) (pTyr176) antibody (ABIN6269655) ABIN6269656 at 1/100 staining mouse heart tissue sections by IHC-P. The tissue was fo...