PDPK1 Antikörper (3-phosphoinositide Dependent Protein Kinase-1) (pThr245)

Details for Product anti-PDPK1 Antibody No. ABIN6269647
Target
Bindungsspezifität
pThr245
25
25
19
18
15
15
15
11
7
6
6
6
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
264
178
173
16
9
8
7
7
5
3
3
3
1
1
1
1
Wirt
Kaninchen
231
30
7
Klonalität
Polyklonal
Konjugat
Dieser PDPK1 Antikörper ist unkonjugiert
15
12
11
10
9
9
9
9
9
7
7
7
7
7
4
4
3
1
1
1
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
142
92
83
47
41
14
13
12
10
6
5
2
1
1
1
Optionen
Immunogen A synthesized peptide derived from human PDK1 around the phosphorylation site of Thr245
Isotyp IgG
Spezifität Phospho-PDK1 (Thr245) Antibody detects endogenous levels of PDK1 only when phosphorylated at Thr245
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung PDK1 (PDPK1 Antibody Abstract)
Hintergrund

Description: Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca2+ entry and Ca2+-activated K+ channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF-kappa-B activation in macrophages. Isoform 3 is catalytically inactive.

Gene: PDPK1

Molekulargewicht 63kDa
Gen-ID 5170
UniProt O15530
Pathways PI3K-Akt Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pThr245) antibody (ABIN6269647) ABIN6269648 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was f...
Image no. 2 for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pThr245) antibody (ABIN6269647) ABIN6269648 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was for...
Image no. 3 for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pThr245) antibody (ABIN6269647) ABIN6269648 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was f...
Image no. 4 for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pThr245) antibody (ABIN6269647) Western blot analysis of Phospho-PDK1 (Thr245) in lysates of HepG2?, using Phospho-PD...