Inositol Polyphosphate Phosphatase-Like 1 (INPPL1) (pTyr671) Antikörper

Details zu Produkt Nr. ABIN6269641
Target
Bindungsspezifität
pTyr671
20
16
16
8
7
3
3
2
2
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
92
82
63
3
3
3
3
2
2
1
1
1
1
Wirt
Kaninchen
88
4
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
6
6
6
6
5
5
5
3
3
3
3
3
3
3
1
1
1
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
39
38
16
13
11
10
4
3
1
Optionen
Immunogen A synthesized peptide derived from human SHIP2 around the phosphorylation site of Tyr671
Isotyp IgG
Spezifität Phospho-SHIP2 (Tyr671) Antibody detects endogenous levels of SHIP2 only when phosphorylated at Tyr671
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung SHIP2 (INPPL1 Antibody Abstract)
Hintergrund

Description: Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Plays a central role in regulation of PI3K-dependent insulin signaling, although the precise molecular mechanisms and signaling pathways remain unclear. While overexpression reduces both insulin-stimulated MAP kinase and Akt activation, its absence does not affect insulin signaling or GLUT4 trafficking. Confers resistance to dietary obesity. May act by regulating AKT2, but not AKT1, phosphorylation at the plasma membrane. Part of a signaling pathway that regulates actin cytoskeleton remodeling. Required for the maintenance and dynamic remodeling of actin structures as well as in endocytosis, having a major impact on ligand-induced EGFR internalization and degradation. Participates in regulation of cortical and submembraneous actin by hydrolyzing PtdIns(3,4,5)P3 thereby regulating membrane ruffling (PubMed:21624956). Regulates cell adhesion and cell spreading. Required for HGF-mediated lamellipodium formation, cell scattering and spreading. Acts as a negative regulator of EPHA2 receptor endocytosis by inhibiting via PI3K-dependent Rac1 activation. Acts as a regulator of neuritogenesis by regulating PtdIns(3,4,5)P3 level and is required to form an initial protrusive pattern, and later, maintain proper neurite outgrowth. Acts as a negative regulator of the FC-gamma-RIIA receptor (FCGR2A). Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Involved in EGF signaling pathway. Upon stimulation by EGF, it is recruited by EGFR and dephosphorylates PtdIns(3,4,5)P3. Plays a negative role in regulating the PI3K-PKB pathway, possibly by inhibiting PKB activity. Down-regulates Fc-gamma-R-mediated phagocytosis in macrophages independently of INPP5D/SHIP1. In macrophages, down-regulates NF-kappa-B-dependent gene transcription by regulating macrophage colony-stimulating factor (M-CSF)-induced signaling. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6. Involved in endochondral ossification.

Gene: INPPL1

Molekulargewicht 139kDa
Gen-ID 3636
UniProt O15357
Pathways Platelet-derived growth Factor Receptor Signaling
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Inositol Polyphosphate Phosphatase-Like 1 (INPPL1) (pTyr671) antibody (ABIN6269641) ABIN6269642 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was f...
Image no. 2 for anti-Inositol Polyphosphate Phosphatase-Like 1 (INPPL1) (pTyr671) antibody (ABIN6269641) ABIN6269642 at 1/100 staining rat brain tissue sections by IHC-P. The tissue was form...
Image no. 3 for anti-Inositol Polyphosphate Phosphatase-Like 1 (INPPL1) (pTyr671) antibody (ABIN6269641) Western blot analysis of Phospho-SHIP2 (Tyr671) in lysates of HeLa?, using Phospho-SH...