CDC34 Antikörper (Cell Division Cycle 34 Homolog (S. Cerevisiae)) (pSer71)

Details for Product anti-CDC34 Antibody No. ABIN6269597
Target
Bindungsspezifität
pSer71
7
5
3
3
2
2
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
58
37
35
4
2
1
1
1
Wirt
Kaninchen
49
10
Klonalität
Polyklonal
Konjugat
Dieser CDC34 Antikörper ist unkonjugiert
4
3
3
3
3
3
3
1
1
Applikation
ELISA, Western Blotting (WB)
35
14
10
6
5
5
5
2
2
1
Optionen
Immunogen A synthesized peptide derived from human CDC34 around the phosphorylation site of Ser71
Isotyp IgG
Spezifität Phospho-CDC34(Ser71) Antibody detects endogenous levels of CDC34 only when phosphorylated at Ser71
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung CDC34 (CDC34 Antibody Abstract)
Hintergrund

Description: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed:22496338). Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes (PubMed:10329681, PubMed:10373550, PubMed:10871850, PubMed:11675391, PubMed:12037680, PubMed:15652359, PubMed:17461777, PubMed:17698585, PubMed:19112177, PubMed:19126550, PubMed:19945379, PubMed:20061386, PubMed:20347421).

Gene: CDC34

Molekulargewicht 26kDa
Gen-ID 997
UniProt P49427
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Cell Division Cycle 34 Homolog (S. Cerevisiae) (CDC34) (pSer71) antibody (ABIN6269597) Western blot analysis of Phospho-CDC34(Ser71) in lysates of RAW 264.7?, using Phospho...