RIPK1 Antikörper (Receptor (TNFRSF)-Interacting serine-threonine Kinase 1) (pTyr384)

Details for Product anti-RIPK1 Antibody No. ABIN6269593
Target
Bindungsspezifität
pTyr384
14
8
8
8
7
7
5
4
4
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
118
47
36
4
4
3
3
2
2
1
1
Wirt
Kaninchen
105
16
2
Klonalität
Polyklonal
Konjugat
Dieser RIPK1 Antikörper ist unkonjugiert
10
6
6
6
6
6
5
4
4
4
2
2
1
1
1
1
1
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
77
44
37
22
16
14
14
4
4
2
1
1
Optionen
Immunogen A synthesized peptide derived from human RIPK1 around the phosphorylation site of Tyr284
Isotyp IgG
Spezifität Phospho-RIPK1(Tyr284) Antibody detects endogenous levels of RIPK1 only when phosphorylated at Tyr284
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung RIPK1 (RIPK1 Antibody Abstract)
Hintergrund

Description: Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.

Gene: RIPK1

Molekulargewicht 76kDa
Gen-ID 8737
UniProt Q13546
Pathways NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Activation of Innate immune Response, Inositol Metabolic Process, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interaktom
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) (pTyr384) antibody (ABIN6269593) Western blot analysis of Phospho-RIPK1(Tyr284) in lysates of HeLa?, using Phospho-RIP...
Image no. 2 for anti-Receptor (TNFRSF)-Interacting serine-threonine Kinase 1 (RIPK1) (pTyr384) antibody (ABIN6269593) ABIN6269594 at 1/100 staining human liver tissue sections by IHC-P. The tissue was fo...