ATG3 Antikörper (ATG3 Autophagy Related 3 Homolog (S. Cerevisiae)) (pTyr18)

Details for Product anti-ATG3 Antibody No. ABIN6269591
Target
Bindungsspezifität
pTyr18
17
15
13
9
7
7
5
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
158
65
64
7
6
4
4
3
3
2
1
1
1
1
1
Wirt
Kaninchen
141
16
2
Klonalität
Polyklonal
Konjugat
Dieser ATG3 Antikörper ist unkonjugiert
14
11
11
6
6
6
5
4
4
4
4
4
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
90
62
33
30
26
26
17
13
9
2
1
1
Optionen
Immunogen A synthesized peptide derived from human ATG3 around the phosphorylation site of Tyr18
Isotyp IgG
Spezifität Phospho-ATG3(Tyr18) Antibody detects endogenous levels of ATG3 only when phosphorylated at Tyr18
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung ATG3 (ATG3 Antibody Abstract)
Hintergrund

Description: E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.

Gene: ATG3

Molekulargewicht 40kDa
Gen-ID 64422
UniProt Q9NT62
Pathways Autophagie
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-ATG3 Autophagy Related 3 Homolog (S. Cerevisiae) (ATG3) (pTyr18) antibody (ABIN6269591) ABIN6269592 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was f...
Image no. 2 for anti-ATG3 Autophagy Related 3 Homolog (S. Cerevisiae) (ATG3) (pTyr18) antibody (ABIN6269591) Western blot analysis of Phospho-ATG3(Tyr18) in lysates of Jurkat , using Phospho-ATG...