Cullin 4B (CUL4B) (pThr48) Antikörper

Details zu Produkt Nr. ABIN6269583
Target
Bindungsspezifität
pThr48
9
9
8
7
7
3
3
3
2
1
1
1
1
1
1
Reaktivität
Human
78
33
30
10
5
4
4
4
4
3
2
2
2
1
1
1
Wirt
Kaninchen
71
7
Klonalität
Polyklonal
Konjugat
Unkonjugiert
6
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
55
24
15
13
12
8
8
3
1
1
1
Optionen
Immunogen A synthesized peptide derived from human CUL4B around the phosphorylation site of Thr48
Isotyp IgG
Spezifität Phospho-CUL4B(Thr48) Antibody detects endogenous levels of CUL4B only when phosphorylated at Thr48
Kreuzreaktivität Human
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung CUL4B (CUL4B Antibody Abstract)
Hintergrund

Description: Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8.

Gene: CUL4B

Molekulargewicht 99kDa
Gen-ID 8450
UniProt Q13620
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Cullin 4B (CUL4B) (pThr48) antibody (ABIN6269583) ABIN6269584 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was f...
Image no. 2 for anti-Cullin 4B (CUL4B) (pThr48) antibody (ABIN6269583) Western blot analysis of Phospho-CUL4B(Thr48) in lysates of 293T?, using Phospho-CUL4...