Embryonic Ectoderm Development (EED) (pThr55) Antikörper

Details zu Produkt Nr. ABIN6269533
Target
Bindungsspezifität
pThr55
7
7
4
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
58
44
28
6
5
5
5
5
4
3
3
3
3
2
Wirt
Kaninchen
52
8
2
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
44
20
13
8
6
4
2
2
2
2
1
1
1
1
Optionen
Immunogen A synthesized peptide derived from human EED around the phosphorylation site of Thr55
Isotyp IgG
Spezifität Phospho-EED(Thr55) Antibody detects endogenous levels of EED only when phosphorylated at Thr55
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung EED (EED Antibody Abstract)
Hintergrund

Description: Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A.

Gene: EED

Molekulargewicht 50kDa
Gen-ID 8726
UniProt O75530
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Embryonic Ectoderm Development (EED) (pThr55) antibody (ABIN6269533) ABIN6269534 at 1/100 staining rat lung tissue sections by IHC-P. The tissue was forma...
Image no. 2 for anti-Embryonic Ectoderm Development (EED) (pThr55) antibody (ABIN6269533) Western blot analysis of Phospho-EED(Thr55) in lysates of HeLa?, using Phospho-EED(Th...
Image no. 3 for anti-Embryonic Ectoderm Development (EED) (pThr55) antibody (ABIN6269533) ABIN6269534 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was f...
Image no. 4 for anti-Embryonic Ectoderm Development (EED) (pThr55) antibody (ABIN6269533) ABIN6269534 at 1/100 staining human heart tissue sections by IHC-P. The tissue was fo...