NEK2 Antikörper (NIMA (Never in Mitosis Gene A)-Related Kinase 2) (pSer170)

Details for Product anti-NEK2 Antibody No. ABIN6269531
Target
Bindungsspezifität
pSer170
15
10
8
8
7
7
6
6
6
5
5
2
2
2
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
111
33
30
3
3
3
2
2
2
2
2
1
1
Wirt
Kaninchen
100
11
3
Klonalität
Polyklonal
Konjugat
Dieser NEK2 Antikörper ist unkonjugiert
9
7
7
6
6
6
3
3
3
3
3
3
1
1
1
1
1
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
90
57
22
14
14
14
8
4
3
2
2
1
Optionen
Immunogen A synthesized peptide derived from human NEK2 around the phosphorylation site of Ser170
Isotyp IgG
Spezifität Phospho-NEK2(Ser170) Antibody detects endogenous levels of NEK2 only when phosphorylated at Ser170
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung NEK2 (NEK2 Antibody Abstract)
Hintergrund

Description: Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC, SGO1, NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (PubMed:24554434). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (PubMed:25704143). Involved in the regulation of centrosome disjunction (PubMed:26220856).

Gene: NEK2

Molekulargewicht 51kDa
Gen-ID 4751
UniProt P51955
Pathways M Phase
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (pSer170) antibody (ABIN6269531) ABIN6269532 at 1/100 staining human heart tissue sections by IHC-P. The tissue was fo...
Image no. 2 for anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (pSer170) antibody (ABIN6269531) ABIN6269532 at 1/100 staining rat heart tissue sections by IHC-P. The tissue was form...
Image no. 3 for anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (pSer170) antibody (ABIN6269531) ABIN6269532 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was f...
Image no. 4 for anti-NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2) (pSer170) antibody (ABIN6269531) Western blot analysis of Phospho-NEK2(Ser170) in lysates of K562, using Phospho-NEK2(...