Cullin 4A Antikörper (CUL4A) (pSer40)

Details for Product anti-CUL4A Antibody No. ABIN6269493
Target
Bindungsspezifität
pSer40
17
9
7
7
4
4
3
3
2
2
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
70
36
7
3
3
2
2
2
2
1
1
1
Wirt
Kaninchen
65
5
Klonalität
Polyklonal
Konjugat
Dieser Cullin 4A Antikörper ist unkonjugiert
6
3
3
3
3
3
3
3
3
2
2
2
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
47
33
12
8
6
5
4
2
2
1
1
1
1
1
Optionen
Immunogen A synthesized peptide derived from human CUL4A around the phosphorylation site of Ser40
Isotyp IgG
Spezifität Phospho-CUL4A(Ser40) Antibody detects endogenous levels of CUL4A only when phosphorylated at Ser40
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung CUL4A (CUL4A Antibody Abstract)
Hintergrund

Description: Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. DCX(DDB2) ubiquitinates histones H3-H4 and is required for efficient histone deposition during replication-coupled (H3.1) and replication-independent (H3.3) nucleosome assembly, probably by facilitating the transfer of H3 from ASF1A/ASF1B to other chaperones involved in histone deposition. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9. DCX(DTL) directs autoubiquitination of DTL.

Gene: CUL4A

Molekulargewicht 88kDa
Gen-ID 8451
UniProt Q13619
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Cullin 4A (CUL4A) (pSer40) antibody (ABIN6269493) ABIN6269494 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was f...
Image no. 2 for anti-Cullin 4A (CUL4A) (pSer40) antibody (ABIN6269493) ABIN6269494 at 1/100 staining human heart tissue sections by IHC-P. The tissue was fo...
Image no. 3 for anti-Cullin 4A (CUL4A) (pSer40) antibody (ABIN6269493) ABIN6269494 at 1/100 staining rat heart tissue sections by IHC-P. The tissue was form...
Image no. 4 for anti-Cullin 4A (CUL4A) (pSer40) antibody (ABIN6269493) Western blot analysis of Phospho-CUL4A(Ser40) in lysates of HeLa?, using Phospho-CUL4...