SKP1 Antikörper (S-Phase Kinase-Associated Protein 1) (pThr131)

Details for Product anti-SKP1 Antibody No. ABIN6269479
Target
Bindungsspezifität
pThr131
9
9
9
7
4
3
3
2
2
2
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
84
44
41
4
2
2
2
1
1
1
1
1
1
Wirt
Kaninchen
75
9
Klonalität
Polyklonal
Konjugat
Dieser SKP1 Antikörper ist unkonjugiert
7
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
Applikation
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
57
40
16
13
12
7
6
5
4
2
1
1
1
Optionen
Immunogen A synthesized peptide derived from human SKP1A around the phosphorylation site of Thr131
Isotyp IgG
Spezifität Phospho-SKP1A(Thr131) Antibody detects endogenous levels of SKP1A only when phosphorylated at Thr131
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung SKP1A (SKP1 Antibody Abstract)
Hintergrund

Description: Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and probably NFKB2 (PubMed:25704143). SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22', the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) directs ubiquitination of TTI1 and TELO2. SCF(FBXO10) directs ubiquitination of BCL2.

Gene: SKP1

Molekulargewicht 19kDa
Gen-ID 6500
UniProt P63208
Pathways Zellzyklus, Hedgehog Signalweg, Mitotic G1-G1/S Phases
Applikations-hinweise WB 1:500-1:2000, IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-S-Phase Kinase-Associated Protein 1 (SKP1) (pThr131) antibody (ABIN6269479) Western blot analysis of Phospho-SKP1A(Thr131) in lysates of COS7, using Phospho-SKP1...
Image no. 2 for anti-S-Phase Kinase-Associated Protein 1 (SKP1) (pThr131) antibody (ABIN6269479) ABIN6269480 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was f...
Image no. 3 for anti-S-Phase Kinase-Associated Protein 1 (SKP1) (pThr131) antibody (ABIN6269479) ABIN6269480 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was f...
Image no. 4 for anti-S-Phase Kinase-Associated Protein 1 (SKP1) (pThr131) antibody (ABIN6269479) ABIN6269480 at 1/100 staining rat kidney tissue sections by IHC-P. The tissue was for...