GAPDH Antikörper (Glyceraldehyde-3-Phosphate Dehydrogenase)

Details for Product anti-GAPDH Antibody No. ABIN6269458
Target
Reaktivität
Huhn, Rind (Kuh), Ziege, Human, Affe, Maus, Schwein, Ratte, Zebrafisch (Danio rerio)
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Wirt
Kaninchen
208
112
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11
1
Klonalität
Polyklonal
Konjugat
Dieser GAPDH Antikörper ist unkonjugiert
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Applikation
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
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Optionen
Immunogen A synthesized peptide derived from human GAPDH
Isotyp IgG
Spezifität GAPDH antibody detects endogenous levels of total GAPDH
Kreuzreaktivität Huhn, Rind (Kuh), Ziege, Human, Affe, Maus, Schwein, Ratte (Rattus), Zebrafisch (Danio rerio)
Reinigung The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Target
Andere Bezeichnung GAPDH (GAPDH Antibody Abstract)
Hintergrund

Description: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Gene: GAPDH

Molekulargewicht 37kDa
Gen-ID 2597
UniProt P04406
Applikations-hinweise WB 1:1000-1:5000 IHC 1:200 IF 1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (ABIN6269458) ABIN6269460 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue ...
Image no. 2 for anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (ABIN6269458) ABIN6269460 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue ...
Image no. 3 for anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (ABIN6269458) Western blot analysis of GAPDH expression in HeLa whole cell lysates,The lane on the ...
Image no. 4 for anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (ABIN6269458) Western blot analysis of extracts from rat brain, mouse muscle, rat liver, using GAPD...
Image no. 5 for anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (ABIN6269458) Western blot analysis of extracts of various sample,using GAPDH antibody.
Image no. 6 for anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) antibody (ABIN6269458) ABIN6269460 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized...
Produkt verwendet in: Sun, Xu, Yi, Chen, Wu, Cao, Zhou, Jiang, Zhang: "Role of 5-HT1A receptor in insular cortex mediating stress - induced visceral sensory dysfunction." in: Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society, Vol. 28, Issue 7, pp. 1104-13, 2018 (PubMed).

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Tian, Feng, Han, Wu, Lv, Shen, Li, Zhang, Liu: "Magnolol Alleviates Inflammatory Responses and Lipid Accumulation by AMP-Activated Protein Kinase-Dependent Peroxisome Proliferator-Activated Receptor α Activation." in: Frontiers in immunology, Vol. 9, pp. 147, 2018 (PubMed).

Hu, Cheng, Huang, Cai, Lian, Wang, Gao: "Effects of Xin-Ji-Er-Kang on heart failure induced by myocardial infarction: Role of inflammation, oxidative stress and endothelial dysfunction." in: Phytomedicine : international journal of phytotherapy and phytopharmacology, Vol. 42, pp. 245-257, 2018 (PubMed).

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Liu, Du, Zhang, Lv, Xiong, Zhang, Li, He, Li, Liu: "Autophagy contributes to hypoxia-induced epithelial to mesenchymal transition of endometrial epithelial cells in endometriosis." in: Biology of reproduction, 2018 (PubMed).

Li, Xiao, Yang, Qin, Gao, Liu, Zhou: "Parthenolide attenuated bleomycin-induced pulmonary fibrosis via the NF-κB/Snail signaling pathway." in: Respiratory research, Vol. 19, Issue 1, pp. 111, 2018 (PubMed).

Ji, Zheng, Liu, Ren, Gao, Zhang, Li: "Resveratrol promotes oxidative stress to drive DLC1 mediated cellular senescence in cancer cells." in: Experimental cell research, Vol. 370, Issue 2, pp. 292-302, 2018 (PubMed).

Xu, Chen, Wang, Yang, Liu, Cheng, Gao, Liu, Xiao: "Involvement of insulin signalling pathway in methamphetamine-induced hyperphosphorylation of Tau." in: Toxicology, Vol. 408, pp. 88-94, 2018 (PubMed).

Meng, Chen, Lei, Han, Zhong, Wang, Liu, Gao, Zhang, Tan, Liu, Zhou, Sun, Yang: "Hsp90β promotes aggressive vasculogenic mimicry via epithelial-mesenchymal transition in hepatocellular carcinoma." in: Oncogene, 2018 (PubMed).

Gao, Zheng, Li, Ran, Sun, Yuan, Xiao: "Aberrant hypomethylation and overexpression of the eyes absent homologue 2 suppresses tumor cell growth of human lung adenocarcinoma cells." in: Oncology reports, Vol. 34, Issue 5, pp. 2333-42, 2017 (PubMed).

Wang, Xi, Sun, Zhang, Jiang, He, Li: "CDK3 is a major target of miR-150 in cell proliferation and anti-cancer effect." in: Experimental and molecular pathology, Vol. 102, Issue 2, pp. 181-190, 2017 (PubMed).

Liu, Liu, Luo, Liu, Luo, Tao, Wu, Lu, Jin, Zhao, Zou: "dNK derived IFN-γ mediates VSMC migration and apoptosis via the induction of LncRNA MEG3: A role in uterovascular transformation." in: Placenta, Vol. 50, pp. 32-39, 2017 (PubMed).

Pan, Zhang, Zhao, Zhou, Wang, Deng: "The cardiac glycoside oleandrin induces apoptosis in human colon cancer cells via the mitochondrial pathway." in: Cancer chemotherapy and pharmacology, Vol. 80, Issue 1, pp. 91-100, 2017 (PubMed).