HMGB1 Antikörper (High Mobility Group Box 1)

Details for Product anti-HMGB1 Antibody No. ABIN6269456
Target
  • HMG1
  • HMG3
  • SBP-1
  • DEF
  • HMG-1
  • Hmg1
  • amphoterin
  • p30
  • hmgb1
  • ik:tdsubc_1a5
  • wu:fb23c02
  • xx:tdsubc_1a5
  • zgc:56110
  • zgc:77104
  • hmg-1
  • hmg3
  • sbp-1
  • hmg1
  • HMGB1
  • Ac2-008
  • high mobility group box 1
  • high-mobility group box 1
  • high mobility group box 1a
  • high mobility group box 1 L homeolog
  • high mobility group protein B1
  • HMGB1
  • Hmgb1
  • hmgb1
  • hmgb1a
  • hmgb1.L
  • LOC100359149
Reaktivität
Human, Maus, Ratte
209
108
97
18
17
14
12
9
9
9
9
6
5
4
4
3
2
2
1
1
Wirt
Kaninchen
168
53
2
2
2
1
1
Klonalität
Polyklonal
Konjugat
Dieser HMGB1 Antikörper ist unkonjugiert
21
17
10
8
8
6
4
4
4
4
4
4
1
1
1
1
1
1
Applikation
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
179
98
72
45
36
35
21
13
12
8
7
4
3
2
1
1
1
1
1
Optionen
Immunogen A synthesized peptide derived from human HMGB1
Isotyp IgG
Spezifität HMGB1 antibody detects endogenous levels of total HMGB1
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Target
Andere Bezeichnung HMGB1 (HMGB1 Antibody Abstract)
Hintergrund

Description: Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury (PubMed:27362237). Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance (PubMed:23519706, PubMed:23446148, PubMed:23994764, PubMed:25048472). Has proangiogdenic activity (By similarity). May be involved in platelet activation (By similarity). Binds to phosphatidylserine and phosphatidylethanolamide (By similarity). Bound to RAGE mediates signaling for neuronal outgrowth (By similarity). May play a role in accumulation of expanded polyglutamine (polyQ) proteins such as huntingtin (HTT) or TBP (PubMed:23303669, PubMed:25549101).

Gene: HMGB1

Molekulargewicht 24kDa
Gen-ID 3146
UniProt P09429
Pathways p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration
Applikations-hinweise WB 1:500-1:2000 IHC 1:200, IF/ICC 1:100-1:500
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-High Mobility Group Box 1 (HMGB1) antibody (ABIN6269456) Western blot analysis of ABIN6269457 expression in various lysates
Image no. 2 for anti-High Mobility Group Box 1 (HMGB1) antibody (ABIN6269456) ABIN6269457 at 1/200 staining human lymphoca tissue sections by IHC-P. The tissue was...
Image no. 3 for anti-High Mobility Group Box 1 (HMGB1) antibody (ABIN6269456) HMGB1 Antibody for IHC in human testis
Image no. 4 for anti-High Mobility Group Box 1 (HMGB1) antibody (ABIN6269456) ABIN6269457 at 1/200 staining human lymphoca tissue sections by IHC-P. The tissue was...
Image no. 5 for anti-High Mobility Group Box 1 (HMGB1) antibody (ABIN6269456) Western blot analysis of HMGB1 expression in Mouse liver tissue.
Image no. 6 for anti-High Mobility Group Box 1 (HMGB1) antibody (ABIN6269456) ABIN6269457 at 1/50 staining human lymphoca tissue sections by IHC-P. The tissue was ...
Image no. 7 for anti-High Mobility Group Box 1 (HMGB1) antibody (ABIN6269456) ABIN6269457 at 1/200 staining human lymphoca tissue sections by IHC-P. The tissue was...
Image no. 8 for anti-High Mobility Group Box 1 (HMGB1) antibody (ABIN6269456) Western blot analysis of HMGB1 expression in HepG2 whole cell lysates,The lane on the...
Image no. 9 for anti-High Mobility Group Box 1 (HMGB1) antibody (ABIN6269456) ABIN6269457 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized...
Image no. 10 for anti-High Mobility Group Box 1 (HMGB1) antibody (ABIN6269456) ABIN6269457 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized...
Produkt verwendet in: Ma, Wang, Wu, Chen, Wang, Yang: "Seawater immersion aggravates burn-associated lung injury and inflammatory and oxidative-stress responses." in: Burns : journal of the International Society for Burn Injuries, Vol. 43, Issue 5, pp. 1011-1020, 2018 (PubMed).

Wu, Xu, Xu, Zheng, Chen, Jiao: "Study on the mechanism of JAK2/STAT3 signaling pathway-mediated inflammatory reaction after cerebral ischemia." in: Molecular medicine reports, Vol. 17, Issue 4, pp. 5007-5012, 2018 (PubMed).

Wu, Zheng, Bai, Xia, Zhang, Guo: "HMGB1/RAGE axis mediates the apoptosis, invasion, autophagy, and angiogenesis of the renal cell carcinoma." in: OncoTargets and therapy, Vol. 11, pp. 4501-4510, 2018 (PubMed).

Pavlyashik, Zharikov, Kiselev: "[Comparative estimation of antilithogenic activity of porcine kidney derived biomedical substance and sodium citrate in experimental urolithiasis]." in: Urologiia (Moscow, Russia : 1999), Issue 2, pp. 24-27, 2017 (PubMed).

Wang, Sun, Li, Deng, Zeng, Tao, Wang, Guan, Zhao: "Urinary MCP-1、HMGB1 increased in calcium nephrolithiasis patients and the influence of hypercalciuria on the production of the two cytokines." in: Urolithiasis, Vol. 45, Issue 2, pp. 159-175, 2016 (PubMed).