TLR4 Antikörper (Toll-Like Receptor 4)

Details for Product anti-TLR4 Antibody No. ABIN6269449
Target
Reaktivität
Human, Maus, Ratte
256
118
88
39
39
37
31
20
10
2
2
1
1
Wirt
Kaninchen
188
107
13
5
Klonalität
Polyklonal
Konjugat
Dieser TLR4 Antikörper ist unkonjugiert
32
30
17
8
7
7
5
5
4
4
4
4
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
174
148
91
64
53
46
38
34
27
26
21
16
9
9
8
7
4
2
1
1
1
1
Optionen
Immunogen A synthesized peptide derived from human TLR4
Isotyp IgG
Spezifität TLR4 antibody detects endogenous levels of total TLR4
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Target
Andere Bezeichnung TLR4 (TLR4 Antibody Abstract)
Hintergrund

Description: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:27022195). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:9237759, PubMed:10835634, PubMed:27022195). Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni2+. Responses triggered by Ni2+ require non-conserved histidines and are, therefore, species-specific (PubMed:20711192). Both M.tuberculosis HSP70 (dnaK) and HSP65 (groEL-2) act via this protein to stimulate NF-kappa-B expression (PubMed:15809303). In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- (PubMed:23880187). Stimulation of monocytes in vitro with M.tuberculosis PstS1 induces p38 MAPK and ERK1/2 activation primarily via TLR2, but also partially via this receptor (PubMed:16622205).

Gene: TLR4

Molekulargewicht 100kDa
Gen-ID 7099
UniProt O00206
Pathways TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades
Applikations-hinweise WB 1:500-1:2000 IHC 1:50-1:200 IF 1:100-1:500
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN6269449) Western blot analysis of TLR4 expression in Hela whole cell lysates,The lane on the l...
Image no. 2 for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN6269449) IHC analysis of TLR4 in mouse kidney tissue
Image no. 3 for anti-Toll-Like Receptor 4 (TLR4) antibody (ABIN6269449) ABIN6269450 staining U-2 OS cells by IF/ICC. The sample were fixed with PFA and perme...
Produkt verwendet in: Zhang, Fei, Tao, Li, Shen, Wang, Liu, Xu: "Neuroprotective Effect of Modified Xijiao Dihuang Decoction against Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury in PC12 Cells: Involvement of TLR4-MyD88/NF-κB Signaling Pathway." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2017, pp. 3848595, 2018 (PubMed).

Huang, Chen, Zhang, Li, Chen, Yang, Liang, Zhu, Xu: "Neuroprotective Effect of Curcumin Against Cerebral Ischemia-Reperfusion Via Mediating Autophagy and Inflammation." in: Journal of molecular neuroscience : MN, Vol. 64, Issue 1, pp. 129-139, 2018 (PubMed).

Liu, Gao, Wang, Yang, Huo, Tian: "Effect of stromal cell-derived factor-1 on myocardial apoptosis and cardiac function recovery in rats with acute myocardial infarction." in: Experimental and therapeutic medicine, Vol. 12, Issue 5, pp. 3282-3286, 2016 (PubMed).