BRISC and BRCA1 A Complex Member 1 (BABAM1) (pSer29) Antikörper

Details zu Produkt Nr. ABIN6268202
Target
Bindungsspezifität
pSer29
10
9
7
2
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
29
3
2
2
2
2
1
1
Wirt
Kaninchen
25
2
1
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
2
2
2
2
2
2
Applikation
ELISA, Western Blotting (WB)
28
19
17
4
2
1
1
1
Optionen
Immunogen A synthesized peptide derived from human MERIT40 around the phosphorylation site of Ser29
Isotyp IgG
Spezifität Phospho-MERIT40 (Ser29) Antibody detects endogenous levels of MERIT40 only when phosphorylated at Ser29
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung MERIT40 (BABAM1 Antibody Abstract)
Hintergrund

Description: Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates (PubMed:24075985, PubMed:26195665). In these 2 complexes, it is probably required to maintain the stability of BABAM2 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36 component. The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed:26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1, deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression (PubMed:24075985). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed:24075985).

Gene: BABAM1

Molekulargewicht 40kDa
Gen-ID 29086
UniProt Q9NWV8
Pathways Positive Regulation of Response to DNA Damage Stimulus
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-BRISC and BRCA1 A Complex Member 1 (BABAM1) (pSer29) antibody (ABIN6268202) Western blot analysis of Phospho-MERIT40 (Ser29) in lysates of A-431?, using Phospho-...