ARHGEF2 Antikörper (rho/rac Guanine Nucleotide Exchange Factor (GEF) 2) (pSer886)

Details for Product anti-ARHGEF2 Antibody No. ABIN6268172
Target
Bindungsspezifität
pSer886
15
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
67
49
46
4
3
2
2
2
1
1
1
Wirt
Kaninchen
62
5
2
Klonalität
Polyklonal
Konjugat
Dieser ARHGEF2 Antikörper ist unkonjugiert
3
3
3
3
3
3
3
1
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
44
21
15
10
9
6
6
4
2
2
1
Optionen
Immunogen A synthesized peptide derived from human GEF-H1 around the phosphorylation site of Ser886
Isotyp IgG
Spezifität Phospho-GEF-H1 (Ser886) Antibody detects endogenous levels of GEF-H1 only when phosphorylated at Ser886
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung GEF-H1 (ARHGEF2 Antibody Abstract)
Hintergrund

Description: Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed:9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans, acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability (By similarity). Involved in neuronal progenitor cell division and differentiation (PubMed:28453519). Involved in the migration of precerebellar neurons (By similarity).

Gene: ARHGEF2

Molekulargewicht 120kDa
Gen-ID 9181
UniProt Q92974
Pathways Negative Regulation of intrinsic apoptotic Signaling
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-rho/rac Guanine Nucleotide Exchange Factor (GEF) 2 (ARHGEF2) (pSer886) antibody (ABIN6268172) Western blot analysis of Phospho-GEF-H1 (Ser886) in lysates of 293T, using Phospho-GE...