TP53BP1 Antikörper (Tumor Protein P53 Binding Protein 1) (pSer1618)

Details for Product anti-TP53BP1 Antibody No. ABIN6268126
Target
Bindungsspezifität
pSer1618
26
19
17
12
8
6
5
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Ratte
140
82
57
12
4
4
4
3
2
2
2
2
1
1
1
Wirt
Kaninchen
126
12
2
1
Klonalität
Polyklonal
Konjugat
Dieser TP53BP1 Antikörper ist unkonjugiert
9
9
6
6
6
6
6
2
2
2
2
2
2
2
Applikation
ELISA, Western Blotting (WB)
75
46
43
30
30
26
20
16
8
5
4
3
3
1
1
Optionen
Immunogen A synthesized peptide derived from human 53BP1 around the phosphorylation site of Ser1618
Isotyp IgG
Spezifität Phospho-53BP1 (Ser1618) Antibody detects endogenous levels of 53BP1 only when phosphorylated at Ser1618
Kreuzreaktivität Human, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung 53BP1 (TP53BP1 Antibody Abstract)
Hintergrund

Description: Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:22553214, PubMed:23333306, PubMed:17190600, PubMed:21144835, PubMed:28241136). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23727112, PubMed:23333306). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136). Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23760478, PubMed:28241136, PubMed:17190600). Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425). Participates to the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112).

Gene: TP53BP1

Molekulargewicht 450kDa
Gen-ID 7158
UniProt Q12888
Pathways DNA Reparatur
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (pSer1618) antibody (ABIN6268126) Western blot analysis of Phospho-53BP1 (Ser1618) in lysates of HeLa thymidine nocod...