IRAK1 Antikörper (Interleukin-1 Receptor-Associated Kinase 1) (pThr209)

Details for Product anti-IRAK1 Antibody No. ABIN6268092
Target
Bindungsspezifität
pThr209
33
19
16
15
15
15
15
14
10
10
9
9
9
8
8
7
4
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
172
110
83
2
1
1
Wirt
Kaninchen
176
25
2
Klonalität
Polyklonal
Konjugat
Dieser IRAK1 Antikörper ist unkonjugiert
13
9
9
7
7
7
7
7
7
5
5
5
4
4
4
4
4
Applikation
ELISA, Western Blotting (WB)
127
91
52
38
35
22
19
18
10
5
3
2
2
1
Optionen
Immunogen A synthesized peptide derived from human IRAK1 around the phosphorylation site of Thr209
Isotyp IgG
Spezifität Phospho-IRAK1 (Thr209) Antibody detects endogenous levels of IRAK1 only when phosphorylated at Thr209
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung IRAK1 (IRAK1 Antibody Abstract)
Hintergrund

Description: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.

Gene: IRAK1

Molekulargewicht 77kDa
Gen-ID 3654
UniProt P51617
Pathways NF-kappaB Signalweg, TLR Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Interleukin-1 Receptor-Associated Kinase 1 (IRAK1) (pThr209) antibody (ABIN6268092) Western blot analysis of Phospho-IRAK1 (Thr209) in lysates of 293T?, using Phospho-IR...