X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6) (pSer520) Antikörper

Details zu Produkt Nr. ABIN6268018
Target
Bindungsspezifität
pSer520
18
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10
8
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7
5
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2
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Reaktivität
Human, Maus, Ratte
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80
58
17
4
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1
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Wirt
Kaninchen
185
36
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Klonalität
Polyklonal
Konjugat
Unkonjugiert
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Applikation
ELISA, Western Blotting (WB)
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Optionen
Immunogen A synthesized peptide derived from human Ku70 around the phosphorylation site of Ser520
Isotyp IgG
Spezifität Phospho-Ku70 (Ser520) Antibody detects endogenous levels of Ku70 only when phosphorylated at Ser520
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung Ku70 (XRCC6 Antibody Abstract)
Hintergrund

Description: Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.

Gene: XRCC6

Molekulargewicht 70kDa
Gen-ID 2547
UniProt P12956
Pathways DNA Reparatur
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6) (pSer520) antibody (ABIN6268018) Western blot analysis of Phospho-Ku70 (Ser520) in lysates of HeLa, using Phospho-Ku70...