KIT Antikörper (Mast/stem Cell Growth Factor Receptor) (pTyr723)

Details for Product anti-KIT Antibody No. ABIN6268010
Target
Bindungsspezifität
pTyr723
66
65
44
38
32
24
24
24
20
18
17
16
15
15
15
15
15
14
13
12
12
11
10
9
9
9
9
9
9
9
9
9
9
9
9
9
8
8
8
8
8
8
8
8
8
8
8
8
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
830
280
155
38
37
28
22
14
9
5
3
3
2
2
1
Wirt
Kaninchen
689
184
53
8
2
2
Klonalität
Polyklonal
Konjugat
Dieser KIT Antikörper ist unkonjugiert
90
82
70
64
62
51
16
14
13
13
13
12
12
9
9
9
9
4
4
4
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
1
Applikation
ELISA, Western Blotting (WB)
445
412
234
178
116
110
102
71
53
44
40
23
13
11
8
6
6
3
2
2
2
1
1
1
Optionen
Immunogen A synthesized peptide derived from human c-Kit around the phosphorylation site of Tyr723
Isotyp IgG
Spezifität Phospho-c-Kit (Tyr723) Antibody detects endogenous levels of c-Kit only when phosphorylated at Tyr723
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung c-Kit (KIT Antibody Abstract)
Hintergrund

Description: Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

Gene: KIT

Molekulargewicht 120kDa,145kDa
Gen-ID 3815
UniProt P10721
Pathways RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Mast/stem Cell Growth Factor Receptor (KIT) (pTyr723) antibody (ABIN6268010) Western blot analysis of Phospho-c-Kit (Tyr723) in lysates of MO7e SCF 50 ng/ml for 2...