IRAK4 Antikörper (Interleukin-1 Receptor-Associated Kinase 4) (pThr342)

Details for Product anti-IRAK4 Antibody No. ABIN6267964
Target
Bindungsspezifität
pThr342
24
21
15
15
15
9
9
8
8
7
5
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
122
43
32
5
3
3
2
2
2
1
Wirt
Kaninchen
111
15
7
Klonalität
Polyklonal
Konjugat
Dieser IRAK4 Antikörper ist unkonjugiert
9
7
7
5
5
5
4
4
4
4
4
4
2
2
2
2
2
Applikation
ELISA, Western Blotting (WB)
94
63
26
18
16
7
6
5
5
3
1
1
1
Optionen
Immunogen A synthesized peptide derived from human IRAK4 around the phosphorylation site of Thr342
Isotyp IgG
Spezifität Phospho-IRAK4(Thr342) Antibody detects endogenous levels of IRAK4 only when phosphorylated at Thr342
Kreuzreaktivität Human, Maus
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung IRAK4 (IRAK4 Antibody Abstract)
Hintergrund

Description: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed:17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.

Gene: IRAK4

Molekulargewicht 55kDa
Gen-ID 51135
UniProt Q9NWZ3
Pathways NF-kappaB Signalweg, TLR Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) (pThr342) antibody (ABIN6267964) Western blot analysis of Phospho-IRAK4(Thr342) in lysates of ?serum-starved KARPAS-29...