Poly(A) Binding Protein, Cytoplasmic 1 (PABPC1) (pTyr364) Antikörper

Details zu Produkt Nr. ABIN6267886
Target
Bindungsspezifität
pTyr364
15
15
15
2
2
2
2
2
1
1
Reaktivität
Human, Maus, Ratte
38
26
22
3
2
2
2
2
2
2
2
1
1
1
Wirt
Kaninchen
37
2
Klonalität
Polyklonal
Konjugat
Unkonjugiert
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
21
14
8
6
5
5
4
3
2
1
1
1
Optionen
Immunogen A synthesized peptide derived from human PABP1 around the phosphorylation site of Tyr364
Isotyp IgG
Spezifität Phospho-PABP1(Tyr364) Antibody detects endogenous levels of PABP1 only when phosphorylated at Tyr364
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung PABP1 (PABPC1 Antibody Abstract)
Hintergrund

Description: Binds the poly(A) tail of mRNA, including that of its own transcript. May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. Involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons, for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. By binding to long poly(A) tails, may protect them from uridylation by ZCCHC6/ZCCHC11 and hence contribute to mRNA stability (PubMed:25480299). Positively regulates the replication of dengue virus (DENV) (PubMed:26735137).

Gene: PABPC1

Molekulargewicht 71kDa
Gen-ID 26986
UniProt P11940
Pathways SARS-CoV-2 Protein Interaktom
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Poly(A) Binding Protein, Cytoplasmic 1 (PABPC1) (pTyr364) antibody (ABIN6267886) Western blot analysis of Phospho-PABP1(Tyr364) in lysates of HCT116?, using Phospho-P...