RBX1 Antikörper (Ring-Box 1, E3 Ubiquitin Protein Ligase) (pThr9)

Details for Product anti-RBX1 Antibody No. ABIN6267858
Target
Bindungsspezifität
pThr9
10
4
3
3
2
2
1
1
1
1
Reaktivität
Human, Maus, Ratte
47
32
27
6
4
4
4
4
3
3
3
2
1
Wirt
Kaninchen
46
2
Klonalität
Polyklonal
Konjugat
Dieser RBX1 Antikörper ist unkonjugiert
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
31
13
13
10
9
7
7
2
1
1
1
Optionen
Immunogen A synthesized peptide derived from human RBX1 around the phosphorylation site of Thr9
Isotyp IgG
Spezifität Phospho-RBX1(Thr9) Antibody detects endogenous levels of RBX1 only when phosphorylated at Thr9
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung RBX1 (RBX1 Antibody Abstract)
Hintergrund

Description: E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase (CRLs) complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (PubMed:27565346). The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM.

Gene: RBX1

Molekulargewicht 13kDa
Gen-ID 9978
UniProt P62877
Pathways Zellzyklus, M Phase, SARS-CoV-2 Protein Interaktom
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-Ring-Box 1, E3 Ubiquitin Protein Ligase (RBX1) (pThr9) antibody (ABIN6267858) Western blot analysis of Phospho-RBX1(Thr9) in lysates of 293, using Phospho-RBX1(Thr...