NLRP3 Antikörper (NLR Family, Pyrin Domain Containing 3) (pSer295)

Details for Product anti-NLRP3 Antibody No. ABIN6267848
Target
Bindungsspezifität
pSer295
16
16
16
10
9
7
4
4
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
117
44
27
2
2
1
1
1
1
Wirt
Kaninchen
87
22
12
1
Klonalität
Polyklonal
Konjugat
Dieser NLRP3 Antikörper ist unkonjugiert
11
8
8
5
5
5
4
4
4
4
4
4
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
63
59
16
13
11
7
6
5
2
2
2
1
1
Optionen
Immunogen A synthesized peptide derived from human NLRP3 around the phosphorylation site of Ser295
Isotyp IgG
Spezifität Phospho-NLRP3(Ser295) Antibody detects endogenous levels of NLRP3 only when phosphorylated at Ser295
Kreuzreaktivität Human, Maus
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung NLRP3 (NLRP3 Antibody Abstract)
Hintergrund

Description: As the sensor component of the NLRP3 inflammasome, plays a crucial role in innate immunity and inflammation. In response to pathogens and other damage-associated signals, initiates the formation of the inflammasome polymeric complex, made of NLRP3, PYCARD and CASP1 (and possibly CASP4 and CASP5). Recruitment of proCASP1 to the inflammasome promotes its activation and CASP1-catalyzed IL1B and IL18 maturation and secretion in the extracellular milieu. Activation of NLRP3 inflammasome is also required for HMGB1 secretion (PubMed:22801494). The active cytokines and HMGB1 stimulate inflammatory responses. Inflammasomes can also induce pyroptosis, an inflammatory form of programmed cell death. Under resting conditions, NLRP3 is autoinhibited. NLRP3 activation stimuli include extracellular ATP, reactive oxygen species, K+ efflux, crystals of monosodium urate or cholesterol, amyloid-beta fibers, environmental or industrial particles and nanoparticles, cytosolic dsRNA, etc. However, it is unclear what constitutes the direct NLRP3 activator. Activation in presence of cytosolic dsRNA is mediated by DHX33 (PubMed:23871209). Independently of inflammasome activation, regulates the differentiation of T helper 2 (Th2) cells and has a role in Th2 cell-dependent asthma and tumor growth (By similarity). During Th2 differentiation, required for optimal IRF4 binding to IL4 promoter and for IRF4-dependent IL4 transcription. Binds to the consensus DNA sequence 5'-GRRGGNRGAG-3'. May also participate in the transcription of IL5, IL13, GATA3, CCR3, CCR4 and MAF (By similarity).

Gene: NLRP3

Molekulargewicht 118kDa
Gen-ID 114548
UniProt Q96P20
Pathways Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-NLR Family, Pyrin Domain Containing 3 (NLRP3) (pSer295) antibody (ABIN6267848) Western blot analysis of Phospho-NLRP3(Ser295) in lysates of RAW 264.7, using Phospho...