STK3 Antikörper (serine/threonine Kinase 3) (pSer316)

Details for Product anti-STK3 Antibody No. ABIN6267836
Target
Bindungsspezifität
pSer316
12
9
7
5
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
72
35
30
9
7
5
4
4
4
3
3
3
2
1
Wirt
Kaninchen
57
15
1
1
Klonalität
Polyklonal
Konjugat
Dieser STK3 Antikörper ist unkonjugiert
4
2
2
2
2
2
2
2
2
2
2
2
Applikation
ELISA, Western Blotting (WB)
62
28
17
11
6
4
3
3
2
1
1
Optionen
Immunogen A synthesized peptide derived from human MST2 around the phosphorylation site of Ser316
Isotyp IgG
Spezifität Phospho-MST2(Ser316) Antibody detects endogenous levels of MST2 only when phosphorylated at Ser316
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung MST2 (STK3 Antibody Abstract)
Hintergrund

Description: Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38.

Gene: STK3

Molekulargewicht 56kDa
Gen-ID 6788
UniProt Q13188
Pathways Tube Formation
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-serine/threonine Kinase 3 (STK3) (pSer316) antibody (ABIN6267836) Western blot analysis of Phospho-MST2(Ser316) in lysates of HeLa , using Phospho-MST2...