ROCK1 Antikörper (rho-Associated, Coiled-Coil Containing Protein Kinase 1) (pTyr913)

Details for Product anti-ROCK1 Antibody No. ABIN6267814
Target
Bindungsspezifität
pTyr913
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12
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1
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Reaktivität
Human, Maus, Ratte
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64
59
6
4
4
4
1
1
1
Wirt
Kaninchen
82
10
Klonalität
Polyklonal
Konjugat
Dieser ROCK1 Antikörper ist unkonjugiert
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Applikation
ELISA, Western Blotting (WB)
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9
5
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1
Optionen
Immunogen A synthesized peptide derived from human ROCK1 around the phosphorylation site of Tyr913
Isotyp IgG
Spezifität Phospho-ROCK1(Tyr913) Antibody detects endogenous levels of ROCK1 only when phosphorylated at Tyr913
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Target
Andere Bezeichnung ROCK1 (ROCK1 Antibody Abstract)
Hintergrund

Description: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.

Gene: ROCK1

Molekulargewicht 160kDa
Gen-ID 6093
UniProt Q13464
Pathways Microtubule Dynamics, WNT Signalweg, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling
Applikations-hinweise WB 1:500-1:2000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1) (pTyr913) antibody (ABIN6267814) Western blot analysis of Phospho-ROCK1(Tyr913) in lysates of cos-7, using Phospho-ROC...