FLT4 Antikörper (Fms-Related Tyrosine Kinase 4) (AA 1279-1283)

Details for Product anti-FLT4 Antibody No. ABIN6267804
Target
Bindungsspezifität
AA 1279-1283
21
16
15
7
6
6
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
129
74
39
1
Wirt
Kaninchen
105
44
9
3
1
Klonalität
Polyklonal
Konjugat
Dieser FLT4 Antikörper ist unkonjugiert
14
10
8
7
6
4
3
3
3
3
3
3
2
2
2
2
2
Applikation
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
96
72
60
34
26
26
13
13
8
5
4
3
2
1
1
Optionen
Immunogen Peptide sequence around aa.1279~1283 (L-A-S-E-E) derived from Human VEGFR-3.
Isotyp IgG
Spezifität The antibody detects endogenous level of total VEGFR-3 protein.
Kreuzreaktivität Human, Maus
Reinigung The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Target
Andere Bezeichnung VEGFR3 (FLT4 Antibody Abstract)
Hintergrund

Description: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development. Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers. The secreted isoform 3 may function as a decoy receptor for VEGFC and/or VEGFD and play an important role as a negative regulator of VEGFC-mediated lymphangiogenesis and angiogenesis. Binding of vascular growth factors to isoform 1 or isoform 2 leads to the activation of several signaling cascades, isoform 2 seems to be less efficient in signal transduction, because it has a truncated C-terminus and therefore lacks several phosphorylation sites. Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr-185', and of AKT1 at 'Ser-473'.

Gene: FLT4

Molekulargewicht 160kDa
Gen-ID 2324
UniProt P35916
Pathways RTK Signalweg, Signaling Events mediated by VEGFR1 and VEGFR2, VEGF Signaling
Applikations-hinweise WB 1:500-1:1000 IHC 1:50-1:200 IF 1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and glycerol
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Bilder
Image no. 1 for anti-Fms-Related Tyrosine Kinase 4 (FLT4) (AA 1279-1283) antibody (ABIN6267804) ABIN6267805 at 1/100 staining human Heart muscle sections by IHC-P. The tissue was fo...
Image no. 2 for anti-Fms-Related Tyrosine Kinase 4 (FLT4) (AA 1279-1283) antibody (ABIN6267804) ABIN6267805 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized...
Image no. 3 for anti-Fms-Related Tyrosine Kinase 4 (FLT4) (AA 1279-1283) antibody (ABIN6267804) Western blot analysis of extracts from HUVEC cells using VEGFR-3 Antibody (right) and...