RAC1 Antikörper

Details for Product anti-RAC1 Antibody No. ABIN6267802
Target
Reaktivität
Human, Maus, Ratte
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Wirt
Kaninchen
95
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Klonalität
Polyklonal
Konjugat
Dieser RAC1 Antikörper ist unkonjugiert
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Applikation
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
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Optionen
Immunogen A synthesized peptide derived from human Rac1
Isotyp IgG
Spezifität Rac1 antibody detects endogenous levels of total Rac1
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Target
Hintergrund

Description: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2, this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3.

Gene: RAC1

Molekulargewicht 26 kDa
Gen-ID 5879
UniProt P63000
Pathways Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling
Applikations-hinweise WB: 1:500~1:1000 IF 1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C.Stable for 12 months from date of receipt
Haltbarkeit 12 months
Bilder
Image no. 1 for anti-RAC1 antibody (ABIN6267802) Western blot analysis of extracts from 3T3, using Rac1 Antibody. The lane on the left...