ATM Antikörper (Ataxia Telangiectasia Mutated) (AA 1979-1983)

Details for Product anti-ATM Antibody No. ABIN6267798
Target
Bindungsspezifität
AA 1979-1983
41
16
16
13
11
9
8
8
6
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
213
101
54
9
5
1
Wirt
Kaninchen
166
48
4
3
3
Klonalität
Polyklonal
Konjugat
Dieser ATM Antikörper ist unkonjugiert
12
8
8
8
8
8
8
8
6
4
3
3
2
2
2
2
2
1
Applikation
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
111
67
63
30
28
27
24
21
15
12
11
4
4
3
2
2
1
1
Optionen
Immunogen Peptide sequence around aa.1979~1983 (E-G-S-Q-S) derived from Human ATM.
Isotyp IgG
Spezifität The antibody detects endogenous level of total ATM protein.
Kreuzreaktivität Human, Maus
Reinigung The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Target
Andere Bezeichnung ATM (ATM Antibody Abstract)
Hintergrund

Description: Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response.

Gene: ATM

Molekulargewicht 350kDa
Gen-ID 472
UniProt Q13315
Pathways p53 Signalweg, Apoptose, DNA Reparatur, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus
Applikations-hinweise WB 1:500-1:1000 IHC 1:50-1:200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Bilder
Image no. 1 for anti-Ataxia Telangiectasia Mutated (ATM) (AA 1979-1983) antibody (ABIN6267798) Western blot analysis of extracts from 293T cells using ATM Antibody
Image no. 2 for anti-Ataxia Telangiectasia Mutated (ATM) (AA 1979-1983) antibody (ABIN6267798) ABIN6267799 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized...