E2F2 Antikörper (E2F Transcription Factor 2) (AA 425-429)

Details for Product anti-E2F2 Antibody No. ABIN6267784
Target
Bindungsspezifität
AA 425-429
10
9
9
8
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
77
48
20
6
5
4
4
3
3
2
2
1
Wirt
Kaninchen
69
6
2
Klonalität
Polyklonal
Konjugat
Dieser E2F2 Antikörper ist unkonjugiert
6
4
3
2
2
2
2
2
2
2
2
2
Applikation
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
61
32
13
8
3
3
3
1
1
1
1
1
Optionen
Immunogen Peptide sequence around aa.425~429(L-F-D-S-Y) derived from Human E2F-2.
Isotyp IgG
Spezifität The antibody detects endogenous level of total E2F-2 protein.
Kreuzreaktivität Human, Maus
Reinigung The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Target
Andere Bezeichnung E2F-2 (E2F2 Antibody Abstract)
Hintergrund

Description: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.

Gene: E2F2

Molekulargewicht 45kDa
Gen-ID 1870
UniProt Q14209
Pathways Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication
Applikations-hinweise WB 1:500-1:1000, IF/ICC 1:100-1:500
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Bilder
Image no. 1 for anti-E2F Transcription Factor 2 (E2F2) (AA 425-429) antibody (ABIN6267784) Western blot analysis of extracts from Hela and K562 cells using E2F-2 Antibody
Image no. 2 for anti-E2F Transcription Factor 2 (E2F2) (AA 425-429) antibody (ABIN6267784) ABIN6267785 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilize...
Image no. 3 for anti-E2F Transcription Factor 2 (E2F2) (AA 425-429) antibody (ABIN6267784) Western blot analysis of extracts from 293, using E2F-2 Antibody.