DDX4 Antikörper (DEAD Box Protein 4) (AA 273-277)

Details for Product anti-DDX4 Antibody No. ABIN6267780
Target
Bindungsspezifität
AA 273-277
15
9
9
7
6
4
3
2
2
2
2
1
1
1
1
1
Reaktivität
Human, Maus, Ratte
76
39
26
15
3
3
2
1
1
1
1
1
1
1
Wirt
Kaninchen
65
11
Klonalität
Polyklonal
Konjugat
Dieser DDX4 Antikörper ist unkonjugiert
6
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Applikation
ELISA, Western Blotting (WB)
48
28
15
13
12
6
3
3
2
2
2
2
1
Optionen
Immunogen Peptide sequence around aa.273~277(T-I-L-V-E)derived from Human DDX4.
Isotyp IgG
Spezifität The antibody detects endogenous level of total DDX4 protein.
Kreuzreaktivität Human, Maus, Ratte (Rattus)
Reinigung The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Target
Andere Bezeichnung DDX4 (DDX4 Antibody Abstract)
Hintergrund

Description: ATP-dependent RNA helicase required during spermatogenesis (PubMed:10920202, PubMed:21034600). Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity). Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (By similarity). Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs (By similarity).

Gene: DDX4

Molekulargewicht 80kDa
Gen-ID 54514
UniProt Q9NQI0
Applikations-hinweise WB 1:500-1:1000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Bilder
Image no. 1 for anti-DEAD Box Protein 4 (DDX4) (AA 273-277) antibody (ABIN6267780) Western blot analysis of DDX4 Antibody expression in Hela, 3t3 and k562 cells lysates.
Image no. 2 for anti-DEAD Box Protein 4 (DDX4) (AA 273-277) antibody (ABIN6267780) Western blot analysis of extracts from mouse lung, using DDX4 Antibody.