E2F1 Antikörper (E2F Transcription Factor 1) (AA 430-434)

Details for Product anti-E2F1 Antibody No. ABIN6267774
Target
Bindungsspezifität
AA 430-434
26
26
25
23
15
13
11
10
10
10
9
9
8
8
8
7
7
5
5
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus
259
86
67
7
6
6
6
5
5
3
3
3
2
1
1
1
1
Wirt
Kaninchen
225
27
14
Klonalität
Polyklonal
Konjugat
Dieser E2F1 Antikörper ist unkonjugiert
22
16
16
9
9
9
8
8
8
8
8
8
3
3
3
3
3
Applikation
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
174
120
42
42
33
30
22
20
17
10
9
3
2
2
2
1
Optionen
Immunogen Peptide sequence around aa.430~434(G-D-L-T-P) derived from Human E2F-1
Isotyp IgG
Spezifität The antibody detects endogenous levels of total E2F-1 protein.
Kreuzreaktivität Human, Maus
Reinigung The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Target
Andere Bezeichnung E2F-1 (E2F1 Antibody Abstract)
Hintergrund

Description: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812). Positively regulates transcription of RRP1B (PubMed:20040599).

Gene: E2F1

Molekulargewicht 70kDa
Gen-ID 1869
UniProt Q01094
Pathways p53 Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagie
Applikations-hinweise WB 1:500-1:2000, IF/ICC 1:100-1:500
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Bilder
Image no. 1 for anti-E2F Transcription Factor 1 (E2F1) (AA 430-434) antibody (ABIN6267774) Western blot analysis of extracts from 3T3 cells using E2F-1 Antibody
Image no. 2 for anti-E2F Transcription Factor 1 (E2F1) (AA 430-434) antibody (ABIN6267774) ABIN6267775 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized...